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發布時間：2018/7/27
點擊次數：562
BioChain是高質量加工生物樣品產品和分析服務的先進者，用于推進轉化醫學，伴隨診斷和臨床產品研發。BioChain的產品可用于DNA和RNA測序，PCR和RT-PCR，基因表達分析，DNA和RNA純化，蛋白質提取和純化以及蛋白質表達分析。BioChain的下一代測序一站式服務，從臨床樣品采集，樣品制備，測序到生物信息學分析，將*改變行業。
除了我們*的生物標本和樣品制備業務，BioChain正在進入臨床應用領域，為癌癥和生殖健康提供臨床基因組學診斷產品和臨床試驗服務。BioChain致力于通過持續流程改進計劃以競爭力的價格實現高質量的產品和服務。BioChain建立了全面的質量保證計劃，以滿足客戶的滿意度，并為我們的內部生產設施保持ISO 9001：2015和ISO 13485：2003認證。
cfPure® Cell-Free DNA Extraction Kit cfure無細胞DNA提取試劑盒是一種基于磁性珠的DNA提取試劑盒，旨在從人血漿和血清中分離循環無細胞DNA(Cfdna)。該工具包有一個快速，簡單，和自動 省略友好協議，允許用戶快速提取高質量的cfdna，準備用于下游應用，包括實時PCR、數字PCR和下一代測序。 該試劑盒也是一個強有力的工具液體活檢研究，如早期發現腫瘤，無創產前檢查等。
biochain K5011625說明書
概觀
BioChain的cfPure®無細胞DNA提取試劑盒專為快速有效純化無循環細胞DNA（cfDNA）而設計。該系統利用二氧化硅涂層的順磁顆粒凈化無細胞[...]
描述
BioChain的cfPure®無細胞DNA提取試劑盒專為快速有效純化無循環細胞DNA（cfDNA）而設計。該系統利用二氧化硅涂層的順磁顆粒來純化無細胞DNA，從小于1 mL到大于10 mL的血清或血漿。開發緩沖液以確保有效回收100bp-500bp的DNA片段，以大限度地回收無細胞DNA。回收的DNA適用于廣泛的下游應用，例如亞硫酸氫鹽測序，NGS和qPCR。
從血漿，血清和尿液中提取高質量的無細胞DNA，用于新一代測序（NGS），亞硫酸氫鹽測序，PCR，qPCR，ddPCR和其他要求苛刻的應用
•用途廣泛： 從單管或24至96孔板中提取<1mL至> 10 mL血清或血漿
•自動化友好： 在KingFisher™和液體處理儀器上，提取大約需要50分鐘
•時間效率： 手動提取協議只需不到75分鐘
•成本效益： 從1 mL血漿中每次提取的競爭價格<$ 3（250 mL試劑盒的定價）
•洗脫體積： 范圍為15μL至50μL
cfPure®協議快速簡便，允許用戶在一小時或更短的時間內處理多個樣品，使其成為生物標記物篩選的理想選擇。BioChain開發了自己的二氧化硅涂層磁珠技術，旨在在分離過程中有效回收低分子量無細胞DNA。除了易于操作和易于自動化之外，如果您正在凈化少量等離子體，您可以使用更少的磁珠溶液 - 節省您的資金。成功分析cfDNA的關鍵是確保有效純化較小的DNA片段（100-500 bp）。如圖1所示，cfPure®無細胞DNA提取試劑盒可有效回收這些重要的小片段。
Cfpure旨在成為可擴展的套件（圖2）。這意味著您可以僅使用cfDNA提取所需的珠子和試劑的數量。這也意味著每個試劑盒的提取次數取決于您希望從中純化DNA的樣品量。例如，如果您正在使用1mL樣品，我們的100mL試劑盒（目錄號K5011610）可處理多達100個樣品，我們的250 mL試劑盒（目錄號K5011610）可處理250個樣品。如果您正在使用10mL樣品，我們的100mL試劑盒（目錄號K5011610）可處理多達10個樣品，我們的250 mL無細胞DNA提取試劑盒（目錄號K5011625）可處理25個樣品。
與用于分離cfDNA的其他技術相比，磁珠提供許多益處。珠子比玻璃纖維過濾器更有效地結合DNA，從而產生更高和更一致的產量。另外，由于不使用過濾器和真空歧管，因此在提取過程中不存在細胞顆粒堵塞這些物品的風險。這種堵塞問題特別涉及富含蛋白質的大體積樣品，例如cfDNA研究中常用的血漿。
cfPure®fCRDNA提取試劑盒提取的cfDNA非常適用于NGS等下游應用。在第三方研究中，使用BioChain的cfPure®（B），競爭對手T（T）和競爭對手Q（Q，基于柱）的cfDNA提取試劑盒從4個供體的10ml人血漿中提取cfDNA。然后為每個樣品構建文庫，并進行全基因組測序。從數據分析來看，三種提取方法并未顯示出顯著的性能差異：插入大小（圖3），定位率，全基因組覆蓋率，替代率和GC偏差（圖4）。
我們優化的基于磁珠的協議使用戶能夠擴展協議，適用于100μl至10 ml的任何樣品體積，具有高度可重復的結果和有效的cfDNA回收率（表1和2）。
cfPure®CellFree DNA提取試劑盒適用于KingFisher™（圖5）和液體處理儀器。使用KingFisher™Flex和BioChain的cfPure®無細胞DNA提取試劑盒可在50分鐘內提取多達96個1 ml樣品，或在1小時內提取24個5 ml樣品。
biochain 2018年部分產品列表：
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L5011001 | PureSil-Silica Magnetic Beads | 1 L | biochain |
L5011010 | PureSil-Silica Magnetic Beads | 10 mL | biochain |
Z1322006 | AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria | 1 kit (48 extractions) | biochain |
Z1322002 | AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml | 1 kit (48 extractions) | biochain |
Z1322001 | AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl | 1 kit (48 extractions) | biochain |
Z1322005 | AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells | 1 kit (48 extractions) | biochain |
Z1322009 | AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples | 1 kit (48 extractions) | biochain |
Z1322010 | AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples | 1 kit (48 extractions) | biochain |
Z1322007 | AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples | 1 kit (48 extractions) | biochain |
Z1322008 | AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum | 1 kit (48 extractions) | biochain |
Z1322004 | AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains | 1 kit (48 extractions) | biochain |
Z1322015 | AnaPrep Total RNA Extraction Kit | 1 kit | biochain |
Z1322017 | AnaPrep Total RNA Extraction Kit with DNase-Treatment | 1 kit | biochain |
Z1322003 | AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma | 1 kit (48 extractions) | biochain |
Z1322011 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A | 1 kit | biochain |
Z1322012 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B | 1 kit | biochain |
T2235090-SB | CancerSeq™ Paraffin Tissue Tumor Block: Colon | 1 Block | biochain |
T2235152-SB | CancerSeq™ Paraffin Tissue Tumor Block: Lung | 1 Block | biochain |
T2235086-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Breast | 5 Slides | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235086-SB | CancerSeq™ Paraffin Tissue Tumor Block: Breast | 1 Block | biochain |
T2235218A-SB | CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma | 1 Block | biochain |
T2235090-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Colon | 5 Slides | biochain |
T2235152-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Lung | 5 Slides | biochain |
T2235218A-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma | 5 Slides | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L1271010 | T4 RNA Ligase 1 | 1000 Units | biochain |
L1271050 | T4 RNA Ligase 1 | 5000 Units | biochain |
L1271500 | T4 RNA Ligase 1 | 50000 Units | biochain |
L1270010 | T4 RNA Ligase 2, truncated | 1000 Units | biochain |
L1270500 | T4 RNA Ligase 2, truncated | 50000 Units | biochain |
KS072012-I | Aligner 1-12 | 12 | biochain |
KS072012-II | Aligner 13-24 | 1 Kit | biochain |
KS072012-III | Aligner 25-36 | 1 Kit | biochain |
KS072012-IV | Aligner 37-48 | 1 Kit | biochain |
KS071012-12 | Gel Breaker Tubes | 12 | biochain |
KS071012-11 | Gel Cutter | 12 | biochain |
KS071012-13 | Gel Filter | 12 | biochain |
KS073012-I | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS073012-II | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS073012-III | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS073012-IV | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS073012 | RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner | 1 Kit | biochain |
KS073012-T | RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
KS074012-I | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS074012-II | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS074012-III | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS074012-IV | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS074012 | RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners | 1 Kit | biochain |
KS074012-T | RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
K5017100 | Blood and Serum DNA Isolation Kit | 1 kit | biochain |
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
K5019100 | FFPE Tissue DNA Extraction Kit | 1 kit | biochain |
K5011350 | FFPE Tissue DNA Extraction Kit – Column | 1 kit | biochain |
K5011450 | FFPE Tissue DNA Extraction Kit — Magnetic Beads | 1 kit | biochain |
K5016005 | Genomic DNA Extraction Kit | 1 kit | biochain |
K5011050 | Saliva DNA Isolation Kit | 1 kit | biochain |
K5018100 | Serum DNA Isolation Kit | 1 kit | biochain |
K5011250 | Stool DNA Isolation Kit | 1 kit | biochain |
K5011150 | Urine DNA Isolation Kit | 1 kit | biochain |
Z5060009 | xMag-Streptavidin Microparticles | 1 ml | biochain |
T6234701-1 | FDA Standard Frozen Tissue Array – Human Adult Normal | 2 slides | biochain |
T6234701-2 | FDA Standard Frozen Tissue Array – Human Adult Normal | 4 slides | biochain |
T6334701-1 | FDA Standard Frozen Tissue Array – Mouse Normal | 2 slides | biochain |
T6334701-2 | FDA Standard Frozen Tissue Array – Mouse Normal | 4 slides | biochain |
T6434701-1 | FDA Standard Frozen Tissue Array – Rat Normal | 2 slides | biochain |
T6434701-2 | FDA Standard Frozen Tissue Array – Rat Normal | 4 slides | biochain |
T6235201 | Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls | 1 slide | biochain |
T6235086-1 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235086-5 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235090-1 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235090-5 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235142-1 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235142-5 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235183-1 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235183-5 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235152-1 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 1 slide | biochain |
T6235152-5 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 5 slides | biochain |
T6734700-G02-1 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G02-5 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6734700-G01-1 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G01-5 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6234700-1 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6234700-5 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6235700-1 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6235700-5 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G02-1 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G02-5 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G01-1 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G01-5 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6134035-1 | Multiple Species Frozen Tissue Array – Brain | 1 slide | biochain |
T6134035-5 | Multiple Species Frozen Tissue Array – Brain | 5 slides | biochain |
T6134149-1 | Multiple Species Frozen Tissue Array – Liver | 1 slide | biochain |
T6134149-5 | Multiple Species Frozen Tissue Array – Liver | 5 slides | biochain |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1：強大的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產品種類齊全，經營超過700多個品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務，貨品一般1-2周到貨。
4：富有競爭力的價格優勢，絕大部分價格有優勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務。客戶包括清華、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發，歡迎合作。
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